1.     What is the main use of fish genetics in aquaculture?
a) Identifying disease-resistant strains of fish b) Enhancing the flavor of
fish  c) Improving the texture of fish
meat   d) None of the above

Answer:
a) Identifying disease-resistant strains of fish

2.     Which of the following biotechnological tools is used
to identify genetic variations in fish populations? a) DNA fingerprinting b)
RNA sequencing c) Western blotting d) ELISA

Answer:
a) DNA fingerprinting

3.     The process of introducing foreign DNA into the genome
of an organism is called: a) Transformation b) Transcription c) Translation d)
Replication

Answer:
a) Transformation

4.     What is the name of the first transgenic fish approved
for human consumption? a) GloFish b) Salmon Express c) AquaAdvantage Salmon d)
Trout Titan

Answer:
c) AquaAdvantage Salmon

5.     Which of the following is not a benefit of genetically
modified fish in aquaculture? a) Enhanced growth rate b) Disease resistance c)
Improved nutrition d) Increased biodiversity

Answer:
d) Increased biodiversity

6.     What is the term used to describe the complete set of
genes in an organism? a) Genome b) Proteome c) Transcriptome d) Metabolome

Answer:
a) Genome

7.     What is the name of the technique used to amplify DNA
sequences in the laboratory? a) PCR b) Gel electrophoresis c) Blotting d)
Hybridization

Answer:
a) PCR

8.     Which of the following is not a type of DNA marker
used in fish genetics research? a) Microsatellites b) SNPs c) RAPDs d) GLPs

Answer:
d) GLPs

9.     What is the main advantage of using genetic markers in
fish breeding programs? a) Increased growth rate b) Disease resistance c)
Increased survival rate d) All of the above

Answer:
d) All of the above

10.         
What is the name
of the enzyme used to cut DNA at specific sites? a) Restriction enzyme b)
Polymerase enzyme c) Ligase enzyme d) Helicase enzyme

Answer:
a) Restriction enzyme

11.         
Which of the
following is not a method used to introduce foreign DNA into fish cells? a)
Microinjection b) Electroporation c) Lipofection d) Western blotting

Answer:
d) Western blotting

12.         
What is the name
of the process used to separate DNA fragments by size using an electric field?
a) Gel electrophoresis b) Southern blotting c) Northern blotting d) Western
blotting

Answer:
a) Gel electrophoresis

13.         
Which of the
following is not a use of fish genetics in aquaculture? a) Selecting broodstock
for breeding programs b) Developing new fish vaccines c) Improving fish welfare
d) Enhancing the color of fish meat

Answer:
d) Enhancing the color of fish meat

14.         
What is the name
of the process used to identify specific DNA sequences using labeled probes? a)
Hybridization b) PCR c) Blotting d) Sequencing

Answer:
a) Hybridization

15.         
Which of the
following is not a type of genetic variation in fish populations? a) Insertions
and deletions b) Nucleotide substitutions c) Gene duplication d) Protein
folding

Answer:
d) Protein folding

16.         
 What is the name of the technique used to
measure gene expression levels in fish? a) RNA sequencing b) Microarray
analysis c) DNA fingerprinting d) Southern blotting

Answer:
b) Microarray analysis

17.         
What is the name
of the protein responsible for cutting DNA during the CRISPR-Cas9 system? a)
Cas9 b) CRISPR c) RNA polymerase d) Ligase

Answer:
a) Cas9

18.         
What is the name
of the process used to selectively amplify a specific region of DNA? a) PCR b)
Blotting c) Sequencing d) Hybridization

Answer:
a) PCR

19.         
Which of the
following is not a potential application of CRISPR-Cas9 in fish biotechnology?
a) Developing disease-resistant fish b) Enhancing fish growth rate c) Creating
new fish species d) Improving the flavor of fish meat

Answer:
d) Improving the flavor of fish meat

20.         
Which of the
following is a potential environmental risk associated with genetically
modified fish? a) Reduced genetic diversity in wild fish populations b)
Increased susceptibility to disease in wild fish populations c) Higher levels
of mercury in fish meat d) None of the above

Answer:
a) Reduced genetic diversity in wild fish populations

Multiple choice questions ( MCQ) in Fish Genetics and Biotechnology

21.         
What is the name
of the process used to determine the order of nucleotides in a DNA molecule? a)
Sequencing b) Hybridization c) PCR d) Microarray analysis

Answer:
a) Sequencing

22.         
What is the name
of the process used to create a complementary DNA copy from RNA? a) Reverse
transcription b) Forward transcription c) PCR d) Blotting

Answer:
a) Reverse transcription

23.         
Which of the
following is not a potential benefit of gene editing in fish biotechnology? a)
Faster and more efficient breeding b) Improved resistance to environmental
stressors c) Enhanced nutritional content of fish meat d) Increased risk of
disease transmission to wild fish populations

Answer:
d) Increased risk of disease transmission to wild fish populations

24.         
What is the name
of the process used to transfer genes from one organism to another using a
vector? a) Gene cloning b) Gene mapping c) Gene expression d) Gene regulation

Answer:
a) Gene cloning

25.         
Which of the
following is not a type of fish biotechnology used in aquaculture? a)
Transgenic fish production b) Gene editing c) Cryopreservation d) None of the
above

Answer:
d) None of the above

26.         
What is the name
of the process used to insert foreign DNA into fish cells using a particle gun?
a) Bioballistics b) Electroporation c) Microinjection d) Lipofection

Answer:
a) Bioballistics

27.         
Which of the
following is not a factor that affects the success of fish breeding programs?
a) Genetic variability b) Broodstock selection c) Environmental conditions d)
Fish feed composition

Answer:
d) Fish feed composition

28.         
What is the name
of the process used to cut and paste DNA sequences from one location to another
within the same genome? a) Transposition b) Transformation c) Transcription d)
Translation

Answer:
a) Transposition

29.         
Which of the
following is not a common method for analyzing the genetic diversity of fish populations?
a) Microsatellite analysis b) Single nucleotide polymorphism (SNP) analysis c)
Mitochondrial DNA sequencing d) Western blot analysis

Answer:
d) Western blot analysis

30.         
What is the name
of the process used to introduce new genetic material into the germline of
fish? a) Somatic cell nuclear transfer b) In vitro fertilization c) Gene
therapy d) Germline engineering

Answer:
d) Germline engineering

Multiple choice questions ( MCQ) in Fish Genetics and Biotechnology

31.         
Which of the
following is not a potential application of gene editing in fish biotechnology?
a) Modifying fish to tolerate high salinity environments b) Creating fish that
produce fewer greenhouse gas emissions c) Enhancing the flavor of fish meat d)
Developing fish that can produce high levels of omega-3 fatty acids

Answer:
c) Enhancing the flavor of fish meat

32.         
What is the name
of the process used to identify specific DNA sequences in a complex mixture of
DNA fragments? a) Hybridization b) Sequencing c) PCR d) Southern blotting

Answer:
d) Southern blotting

33.         
Which of the
following is a potential ethical concern associated with fish biotechnology? a)
Decreased availability of wild-caught fish b) Increased risk of disease
transmission to humans c) Reduced genetic diversity in farmed fish populations
d) All of the above

Answer:
c) Reduced genetic diversity in farmed fish populations

34.         
What is the name
of the process used to remove undesirable genes from a population using
selective breeding? a) Genetic engineering b) Gene editing c) Genetic drift d)
Negative selection

Answer:
d) Negative selection

35.         
Which of the
following is not a potential application of transgenic fish production? a)
Creating fish that are resistant to diseases b) Producing fish with increased
growth rates c) Developing new species of fish d) Enhancing the nutritional
content of fish meat

Answer:
c) Developing new species of fish

36.         
What is the name
of the process used to introduce foreign DNA into fish cells using an
electrical field? a) Bioballistics b) Electroporation c) Microinjection d)
Lipofection

Answer:
b) Electroporation

37.         
Which of the
following is not a potential benefit of fish biotechnology in aquaculture? a)
Reduced environmental impact of fish farming b) Increased fish production for
human consumption c) Improved human health through the consumption of
nutritious fish d) Increased genetic diversity in farmed fish populations

Answer:
d) Increased genetic diversity in farmed fish populations

38.         
What is the name
of the process used to determine the presence or absence of specific DNA
sequences in a sample? a) Sequencing b) Hybridization c) PCR d) Microarray
analysis

Answer:
b) Hybridization

39.         
Which of the
following is not a potential limitation of CRISPR-Cas9 in fish biotechnology?
a) Off-target effects b) Limited availability of Cas9 protein c) High cost of
technology d) Difficulty in delivering the CRISPR-Cas9 system to fish cells

Answer:
b) Limited availability of Cas9 protein

40.         
What is the name
of the process used to analyze genetic variation within and between fish
populations? a) Genotyping b) Gene expression analysis c) Proteomics d)
Metagenomics

Answer:
a) Genotyping

Multiple choice questions ( MCQ) in Fish Genetics and Biotechnology

41.         
Which of the
following is not a potential application of genetic engineering in fish
biotechnology? a) Developing fish that can tolerate low oxygen environments b)
Creating fish that produce biodegradable plastics c) Enhancing the flavor of
fish meat d) Developing fish that can produce high levels of pharmaceuticals

Answer:
c) Enhancing the flavor of fish meat

42.         
Which of the
following is a potential disadvantage of using gene editing in fish
biotechnology? a) Reduced environmental impact of fish farming b) Potential
off-target effects c) Reduced nutritional value of fish meat d) Increased risk
of genetic contamination of wild fish populations

Answer:
b) Potential off-target effects

43.         
Which of the
following is a potential benefit of using genome-wide association studies in
fish biotechnology? a) Identifying genetic markers associated with disease
resistance in fish b) Increasing the genetic diversity of farmed fish
populations c) Creating new species of fish d) Enhancing the flavor of fish
meat

Answer:
a) Identifying genetic markers associated with disease resistance in fish

44.         
What is the name
of the process used to analyze gene expression in fish? a) Genotyping b)
Proteomics c) Transcriptomics d) Metagenomics

Answer:
c) Transcriptomics

45.         
Which of the
following is not a potential application of RNA interference in fish
biotechnology? a) Developing fish that produce fewer greenhouse gas emissions
b) Creating fish that can tolerate high salinity environments c) Enhancing the
nutritional content of fish meat d) Increasing the growth rates of farmed fish

Answer:
c) Enhancing the nutritional content of fish meat

46.         
What is the name
of the process used to identify genetic variation in a population of fish? a)
Genotyping b) Sequencing c) Microarray analysis d) CRISPR-Cas9 editing

Answer:
a) Genotyping

47.         
Which of the
following is not a potential application of bioballistics in fish
biotechnology? a) Introducing foreign DNA into fish cells b) Creating
transgenic fish c) Developing fish that can tolerate low oxygen environments d)
Modifying the genetic makeup of wild fish populations

Answer:
d) Modifying the genetic makeup of wild fish populations

48.         
What is the name
of the process used to create transgenic fish by inserting foreign DNA into the
genome of the fish? a) Somatic cell nuclear transfer b) Germline engineering c)
Gene editing d) Microinjection

Answer:
d) Microinjection

49.         
Which of the
following is not a potential application of gene editing in fish biotechnology?
a) Creating fish that can tolerate high temperatures b) Developing fish that
produce biodegradable plastics c) Enhancing the flavor of fish meat d) Creating
fish that are resistant to diseases

Answer:
c) Enhancing the flavor of fish meat

50.         
What is the name
of the process used to determine the sequence of nucleotides in a sample of
DNA? a) Sequencing b) Genotyping c) Southern blotting d) PCR

Answer:
a) Sequencing

51.         
Which of the
following is not a common technique used in fish genetic engineering? a) RNA
interference b) CRISPR-Cas9 gene editing c) Transposon-mediated gene transfer
d) Microarray analysis

Answer:
d) Microarray analysis

52.         
Which of the
following is a potential disadvantage of using transgenic fish in aquaculture?
a) Increased resistance to disease b) Reduced environmental impact c) Potential
risk of genetic contamination of wild fish populations d) Enhanced nutritional
value of fish meat

Answer:
c) Potential risk of genetic contamination of wild fish populations

53.         
Which of the
following is not a common application of fish biotechnology? a) Developing new
fish species b) Enhancing the growth rates of farmed fish c) Improving the
nutritional content of fish meat d) Reducing the environmental impact of fish
farming

Answer:
a) Developing new fish species

54.         
Which of the following
is not a common method for analyzing gene expression in fish? a) RNA sequencing
b) Microarray analysis c) Polymerase chain reaction (PCR) d) Electrophoresis

Answer:
d) Electrophoresis

55.         
Which of the
following is a potential benefit of using gene editing in fish biotechnology?
a) Enhancing the flavor of fish meat b) Developing fish that can tolerate low
temperatures c) Creating new species of fish d) Increasing the genetic
diversity of farmed fish populations

Answer:
b) Developing fish that can tolerate low temperatures

56.         
What is the name
of the process used to create a transgenic fish with a specific trait, such as
disease resistance? a) Knockout engineering b) Gene targeting c)
Transposon-mediated gene transfer d) Selective breeding

Answer:
b) Gene targeting

57.         
Which of the
following is not a potential application of biotechnology in fish conservation?
a) Studying the genetic diversity of wild fish populations b) Developing
methods for restoring endangered fish populations c) Creating genetically modified
fish for commercial purposes d) Developing techniques for conserving and
preserving fish germplasm

Answer:
c) Creating genetically modified fish for commercial purposes

58.         
Which of the
following is not a common method for analyzing DNA sequences in fish? a) Sanger
sequencing b) Next-generation sequencing c) Restriction fragment length
polymorphism (RFLP) analysis d) Mass spectrometry

Answer:
d) Mass spectrometry

59.         
Which of the
following is a potential benefit of using biotechnology to enhance the
nutritional content of fish meat? a) Reduced environmental impact of fish
farming b) Increased resistance to disease c) Enhanced flavor and texture of
fish meat d) Improved health benefits for consumers

Answer:
d) Improved health benefits for consumers

60.         
Which of the
following is a potential benefit of using CRISPR-Cas9 gene editing in fish
biotechnology? a) Creating new species of fish b) Increasing the genetic
diversity of farmed fish populations c) Developing fish that can tolerate high
temperatures d) Enhancing the color of fish meat

Answer:
c) Developing fish that can tolerate high temperatures

61.         
Which of the
following is a common method for producing transgenic fish? a) Injection of DNA
into fish eggs b) Injection of RNA into fish eggs c) Direct gene transfer into
fish cells d) All of the above

Answer:
d) All of the above

62.         
Which of the
following is a potential benefit of using biotechnology to reduce the
environmental impact of fish farming? a) Increased resistance to disease b)
Improved growth rates of farmed fish c) Reduced reliance on antibiotics and
other medications d) Enhanced flavor and texture of fish meat

Answer:
c) Reduced reliance on antibiotics and other medications

63.         
Which of the
following is not a common method for producing transgenic fish? a) Somatic cell
nuclear transfer b) Embryo microinjection c) Homologous recombination d)
CRISPR-Cas9 gene editing

Answer:
c) Homologous recombination

64.         
Which of the
following is not a potential application of fish biotechnology in aquaculture?
a) Enhancing the disease resistance of farmed fish b) Improving the growth
rates of farmed fish c) Developing new fish species for commercial purposes d)
Reducing the cost of fish production

Answer:
c) Developing new fish species for commercial purposes

65.         
Which of the
following is not a potential benefit of using biotechnology to improve the
nutritional content of fish meat? a) Increased protein content b) Increased
omega-3 fatty acid content c) Reduced mercury content d) Increased growth rates
of farmed fish

Answer:
d) Increased growth rates of farmed fish

66.         
What is the name
of the process used to study the structure and function of genes in fish? a)
Genomics b) Transcriptomics c) Proteomics d) Metabolomics

Answer:
a) Genomics

67.         
Which of the
following is not a common application of gene editing in fish biotechnology? a)
Creating fish that can tolerate low oxygen levels b) Developing fish that can
tolerate high temperatures c) Enhancing the disease resistance of farmed fish d)
Increasing the size of farmed fish

Answer:
d) Increasing the size of farmed fish

68.         
Which of the
following is a potential benefit of using biotechnology to develop new vaccines
for fish? a) Reduced environmental impact of fish farming b) Enhanced growth rates
of farmed fish c) Improved disease resistance of farmed fish d) Increased
nutritional value of fish meat

Answer:
c) Improved disease resistance of farmed fish