PCR based Primer designing methods through Bioinformatics

• Bioinformatics is an emerging scientific discipline that
  uses information technology to organize, analyze and disseminate biological
  information in order to answer complex biological questions (Singh and Kumar,
  2001). It involves solving complex biological problems using tools and computer
  systems. Selection of primers 3 is useful for polymerase chain reaction (PCR),
  oligo hybridization and DNA sequencing. 
• Proper primer design is actually one of
  the most important factors / steps for successful DNA sequencing. Various
  bioinformatics programs are available for selecting primer pairs from a model
  sequence. 
• All computer programs are written primarily to facilitate the design
  process of primers and are not intended to replace the eye of the experienced
  investigator and laboratory experience to assess the suggested primers before
  engaging in their synthesis ( Binas, 2000).
• The optimal primer sequence and the
  appropriate primer concentration are essential for maximum PCR specificity and
  efficiency. 
• A poorly designed primer can cause little or no product due to
  non-specific amplification and / or the formation of a primer dimer, which can
  be competitive enough to suppress product formation.
• There are several online
  tools available to help molecular biologists design effective PCR primers.

• A
  molecule that serves as a starting material for a polymerization process
• Single stranded oligonucleotides that match exactly the beginning and end of
  the DNA template 
• Generated synthetically 
• Individual primer binds
  specifically to the 3 prime end of the target sequence on the appropriate
  strands of DNA.

• It is an “online genomic browser hosted by the
  University of California Santa Cruz (UCSC). 
• It is an interactive website that
  provides access to genome sequence data from a variety of ‘vertebrate and
  invertebrate species 
• It include major organisms, model, integrated into a large
  collection of aligned annotations.

• BLAST uses an algorithm for comparing primary
  biological sequence information such as amino-acid sequences of different proteins. 
• A BLAST search enables a
  researcher to compare a query sequence with a library or database of sequences, and identify library sequences that resemble
  the query sequence above a certain threshold (Oehmen, C. S.; Baxter, D. J. (2013).

• BLAT
  use to search local sequence of Insilco PCR with high similarity,
• In which we
  select high similar sequence rest of other all mention in list
• Resulted image will be show blow mentioned type.

• The use of software in biological applications has given a new dimension to the field of bioinformatics.
• Many different primer design programs are now available.
• Free software is available on the Internet and many universities have established servers on which a user can connect and perform free analyzes of proteins and nucleic acid sequences (Singh and Kumar, 2001). 
• There are several programs available for full DNA and protein analysis, secondary structure predictions, primer design, molecular modeling, development of cloning strategies, plasmid extraction or analysis. restriction enzymes. 
• Biological software development companies and some scientists have also developed algorithms and computer programs (Rychlik and Rhoades.,
  1989)(Lowe et al., 1990)(Lucas et al., 1991)(O’Hara and Venezia., 1991)(Tamura
  et al., 1991).

Picking primers–
click on the ‘pick primers’ button. Output will show save prime with name