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Multiple Choice Questions of Biotechnology OR Objective Questions of Biotechnology

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 Multiple Choice Questions (MCQ) or Objective Questions of 

Biotechnology
Objective Questions of Biotechnology


 

 

 

 

 

1)     
Sugar found in the DNA is…..

 

(a)

Hexose
Sugar

(b)

Octose
Sugar

(c)

Pentose
Sugar

(d)

Tetrose
Sugar

 

2)

Purine N2 Bases have

 

(a)

One ring

(b)

Two ring

(c)

Three ring

(d)

Four Ring

 

3)

RFLP
stands for

 

(a)

Restriction
frequency length polymorphism

(b)

Recombination
fragment length polymorphism

(c)

Restriction
fragment length polymorphism

(d)

Restriction
fragment low polymorphism

 

 

4)

 

Scientific
name of Thymine is

 

(a)

6-aminopurine

(b)

2,4-dioxo-pyrimidine

(c)

2-amino-6
oxopurine

(d)

5-methyl
uracil

 

5)

Synonymous
SNP leads to the

 

(a)

Changes in
the amino acids

(b)

Changes in
the Protein

(c)

No change in
amino acid

(d)

None of these

 

6)

Monomeric
units of DNA is

 

(a)

Nucleotide

(b)

Nucleoside

(c)

Polynucleoside

(d)

Phosphate
Group

 

7)

Which of the
following is a blunt end cutter restriction enzyme

 

(a)

EcoRI

(b)

PstI

(c)

EcoRV

(d)

HindII

 

8)

Two nitrogenous bases are
held together with the help of

 

(a)

Hydrogen Bond

(b)

Peptide Bond

(c)

Glycosidic
Bond

(d)

Phophodiester
Bond

 

9)

The EcoRI enzyme has the recognition site of…..

(a)

5’-GGTACC-3’

(b)

5’-GTTAAC-3’

(c)

5’-CCGGG-3’

(d)

5’-GAATTC-3’

 

 

10)

According to
Watson Crick model the helix diameter of a double stranded DNA is

 

(a)

20 Ǻ

(b)

10 Ǻ

(c)

34 Ǻ

(d)

12 Ǻ

 

11)

Sanger
Sequencing is based on the principle of

(a)

Sequencing by
Synthesis

(b)

Release of
PPi

(c)

Chain
Termination

(d)

Bridge
Amplification

 

12)

DNA’s two
ends can be joined together and sealed by the enzyme………..

(a)

Endonucleases

(b)

Topoisomerases

(c)

Restriction
Enzymes

(d)

Ligases

 

13)

……. type of
restriction enzyme cleave DNA either within the recognition site or very
close to it.

 

(a)

Type I

(b)

Type II

(c)

Type III

(d)

Type IV

 

14)

Genomics is
the study of…..

 

(a)

Complete
Genome

(b)

Complete
Microbes

(c)

Complete
Proteome

(d)

All of these

 

15)

Aqua advantage Atlantic Salmon
received the promoter gene from.

 

(a)

Pacific
Chinook salmon

(b)

Ocean pout

(c)

Chum Salmon

(d)

Coho Salmon

 

16)

AFLP
stands for

(a)

Amplified
Frequency Length Polymorphism

(b)

Amplified
Fragment Length Polymorphism

(c)

Autonomous
Fragment Length Polymorphism

(d)

Amplified
Fragment Low Polymorphism

 

17)

Scientific
name of Uracil is

 

(a)

6-aminopurine

(b)

2,4-dioxo-pyrimidine

(c)

2-amino-6
oxopurine

(d)

2-amino-4-oxo
purine

 

18)

Nom-Synonymous
SNP leads to the

(a)

Changes in
the amino acids

(b)

No changes in
the Protein

(c)

No change in
amino acid

(d)

None of these

 

19)

The 8-10 nucleotide
sequence is of normally.

 

(a)

Primer

(b)

Taq
Polymerase

(c)

Probe

(d)

Vector

 

20)

N2 base and pentose sugar
are held together with the help of

(a)

Hydrogen Bond

(b)

Peptide Bond

(c)

Glycosidic
Bond

(d)

Phophodiester
Bond

 

21)

The EcoRV enzyme has the recognition site of…..

 

(a)

5’-GGTACC-3’

(b)

5’-GAATTC-3’

(c)

5’-CCGGG-3’

(d)

5’-GATATC-3’

           

 

34)

SSR comes
under …………………of the human genome.

 

(a)

Highly
repetitive sequences

(b)

Moderate
repetitive sequences

 

(c)

Low
repetitive sequences

(d)

Unique
sequences

 

 

35)

Multiple
ribosomes translating a single mRNA molecule is called

 

(a)

Polycistrone

(b)

Bridge
Amplification

 

(c)

Polysome

(d)

None of these

 

 

36)

In
mammals…………….. is responsible for the synthesis of mRNA.

 

(a)

RNA
Polymerase I

(b)

RNA
Polymerase II

 

(c)

RNA
Polymerase III

(d)

All of these

 

 

37)

Out of 5
types ………….mRNA involves in splicing and regulation of gene expression.

 

(a)

t-RNA

(b)

snRNA

 

(c)

miRNA

(d)

rRNA

 

 

38)

Translation halts when a
termination codon enters the……………..

 

(a)

P
site

(b)

E
site

 

(c)

A
site

(d)

L
site

 

39)

In Eukaryote the ………………. ribosomal
subunits together form a ribosome with an S value of 80.

 

 

(a)

60S & 20S

(b)

60S & 40S

 

(c)

50S & 30S

(d)

40S & 40S

 

40)

F+
bacteria conjugate with F– bacteria, resulted in….…..

 

 

(a)

One F+
and another F–

(b)

Both bacteria
become F–

 

(c)

Both bacteria
become F+

(d)

None of these

       41)  Father of DNA barcoding is

        a) Alec Jeffery                         b)      Paul Hebert

       c) Bryan Donkin                           d) Oswald Schmiedeberg


     42) The nuclear
DNA marker is               

                     a) RFLP                             b) RAPD

                      

                   c) Microsatellites                 d) All the above

              

              43)  The isoenzyme products of 2 different
alleles at the some locus are known as 

 

a)      Soluble protein                            b) Isoenzyme

c)   Allozymes                                   d) ITS

 

         
   44) Which type of marker
derived mostly from non-coding sequences and are highly

                       Polymorphic

 

a)     
Type II                                                
  b) Type III

                   c) Type I                                                   
d) Type IV

              45)  GFP is

 

          
a)   Gray fluorescent protein                        b) Green fluorescent protein

           c) Greenish fluorescent protein                  d) None of the above

 

           46 Institute of Genomics and
Integrative Biology is located at

a)   
Kolkata                                       b)
cochin

         c) Mumbai                                         d)Delhi

 

             47 First transgenic
plant is

a)     Maize                            
b) cotton

c)Tabacco                            d)  Rice

 

 48.. National Bureau of Fish
Genetic Resources Located at

 

a)      Mumbai             b)cochin  c)Lucknow         d)Delhi

 

49) Preferred gene is set
from mRNA by reverse transcription process and the product is known as

 a) Synthetic DNA                        b) Passenger DNA

 

              c)Complementary DNA                        d)      Random DNA

 

 50 Father of Biotechnology
is

 a)   Oswald Schmiedeberg                 b) Bryan Donkin                          

c) Karoly Ereky                     d)  Paul
Berg

 

 

Basic Multiple Choice or Objective Questions of Biotechnology


Answer Keys

1.    
C

2.    
B

3.    
C

4.     D

5.     C

6.     A

7.     C

8.     A

9.     D

10.                       
A

11.                       
C

12.                       
D

13.                       
B

14.                       
A

15.                       
B

16.                       
B

17.                       
B

18.                       
A

19.                       
C

20.                       
C

21.                       
D

22.                       
A

23.                       
C

24.                       
D

25.                       
D

26.                       
C

27.                       
D

28.                       
C

29.                       
B

30.                       
C

31.                       
C

32.                       
C

33.                       
B

34.                       
A

35.                       
C

36.                       
B

37.                       
B

38.                       
C

39.                       
B

40.                       
C

     41) b
 

    42 ) d  

     43 ) c

     44 ) a  

     45 ) b  

    46 ) d  47) c 48) c   49)  c 50) c            

                            Objective Questions of Biotechnology



Noted: Credit of writing of the above technical questions goes to Dr. Deepak Agarwal, Assistant Professor Institue of fishries, post graguate stuides TNJFU, OMR campuus, Chennai 


 

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